Fig. 6 | Scientific Reports

Fig. 6

From: Irisin activates the AMPK-Beclin1 signaling pathway to regulate pulmonary autophagy induced by CSE + LPS

Fig. 6

Irisin repairs the blocked autophagic flux in CSE + LPS-induced MH-S cells through the AMPK-Beclin1 pathway. (A) Western blotting was used to detect the expression of AMPK, p-AMPK, Beclin1, P62, and LC3II proteins. (n = 3) MH-S cells were treated with CSE2%+LPS(10ug/ml), irisin(20nM), and CC(10µM) for 12 h. (B-F) Intensity of protein bands in different treatments were quantified using Image J software and normalized to GADPH. Data are presented as mean ± SD. Signifcant differences were presented as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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