Fig. 3

Absence of CNTF in culture medium reduces calcium events. (A) Representative images of jRGECO signal in cultures with B/G/C or no GFs at WIC6. Scale bar, 100 μm. (B) Example traces of jRGECO intensity of cultures in B/G/C and no GFs. Grey lines show traces of individual cells and red lines show the average signal in the (FOV). (C) Percentage silent cells in a FOV. N numbers of individual experiments and single neuron observations in brackets: B/G/C: 6(36); no GF: 4(17); BDNF: 4(20); GNDF: 4(21); CNTF: 4(21); B/G/NT3: 6(36); B/G: 6(44); B/C: 6(39); G/C: 6(37). (D) The mean frequency of calcium events of individual neurons in a FOV. N numbers of individual experiments: B/G/C: 6(36); no GF: 4(20); BDNF: 4(21); GNDF: 4(21); CNTF: 4(21); B/G/NT3: 6(36); B/G: 6(44); B/C: 6(38); G/C: 6(37). (E) The mean frequency of network bursts in a FOV, i.e. bursts in which 80% of the neurons are active. (F) Percentage of synchronicity, defined by the percentage cells active at the same time. Bar plots represent the mean ± SEM. Each dot represents an individual neuron. Kruskal-Wallis H test with Dunn’s correction: * P < 0.05, ** P < 0.01, *** P < 0.001. ns = non-significant, P > 0.05.