Fig. 3
METTL3 enhances LINC01315 level via m6A modification in HCC. (A). Forest plot illustrating correlations between LINC01315 expression and multiple m6A regulators (METTL3, METTL14, WTAP, YTHDF1/2/3, ALKBH5, YTHDC1/2, FTO, IGF2BP1/2/3) in HCC tissues. (B). Spearman correlation analysis of LINC01315 with selected regulators (METTL3, WTAP, YTHDF1/2/3, YTHDC1, IGF2BP1, IGFB2P2). (C). RT-PCR showing LINC01315 levels in HepG2 and Huh7 cells after siRNA-mediated knockdown of METTL3, METTL14, WTAP, FTO, or ALKBH5. (D). Predicted m6A sites in LINC01315 identified using the SRAMP database. (E). MeRIP-qPCR showing reduced m6A enrichment of LINC01315 after METTL3 knockdown in HepG2 and Huh7 cells. The obtained data have been reported as the mean accompanied by the standard deviation (SD) (n = 3).p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).
