Fig. 4
YTHDF1 promotes the stability of LINC01315 in HCC cells. (A). RT-PCR analysis of LINC01315 expression in both HCC cell lines after knockdown of YTHDF1, YTHDF2, YTHDF3, YTHDC1, YTHDC2, IGF2BP1, IGF2BP2, or IGF2BP3 (si-NC as control). (B). RIP-qPCR assessing the binding of LINC01315 with YTHDC1/2, YTHDF1/2/3, and IGF2BP1/2/3 in both HCC cell lines (anti-IgG as control). (C). RNA degradation assay showing decay curves and half-life of LINC01315 in both HCC cell lines after YTHDF1 knockdown (si-NC vs. si-YTHDF1). The obtained data have been reported as the mean accompanied by the standard deviation (SD) (n = 3). p < 0.001 (***), p < 0.01 (**), p < 0.05 (*).
