Fig. 1 | Scientific Reports

Fig. 1

From: Ligand non-competitive GITR antibody prevents formation of the obligatory signal-triggering GITRL: GITR stoichiometry

Fig. 1The alternative text for this image may have been generated using AI.

Discovery and characterization of ligand non-competitive NFκB neutralizing anti-GITR antibody. (A) Discovery of ligand competitive and non-competitive antibodies from mice immunization campaign measured by antibody mediated % inhibition of human GITRL binding to recombinant human GITR protein via competition ELISA. (B) Recombinant GITRL protein induces NFκB signaling in GITR-NFκB-Luciferase-Jurkat cell line. (C) Bivalent ligand non-competitive anti-GITR antibody (hGITR-nc-Ab-#1) neutralizes recombinant GITRL protein induced NFκB signaling. (D) Monovalent one-armed ligand non-competitive anti-GITR antibody (hGITR-nc-Ab-#1-OA) also neutralizes recombinant GITRL protein induced NFκB signaling.

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