Fig. 3 | Scientific Reports

Fig. 3

From: Ligand non-competitive GITR antibody prevents formation of the obligatory signal-triggering GITRL: GITR stoichiometry

Fig. 3

High affinity anti-GITR antibody hGITR-nc-Ab-#2 neutralizes GITRL signaling and prevents GITRL mediated cytokine release. (A) hGITR-nc-Ab-#2 binds to GITR-Jurkat-NF-κB-Luc cell line with an EC50 of 38 pM by FACS. (B) hGITR-nc-Ab-#2 neutralizes recombinant GITRL mediated NFKb signaling in GITR-Jurkat-NF-κB-Luc cell line with IC50 of ~ 130pM. (C) Human PBMCs were stimulated for 72 h with TCR stimulation, cytokine cocktail, and recombinant GITRL. hGITR-nc-Ab-#2 inhibits GITRL-mediated secretion of IL-9 and IL-13.

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