Fig. 4

The gel electrophoresis results of One-step PCR and nested PCR targeted 18S rRNA gene of Acanthamoeba in corneal samples of AK patients. (A) The PCR product of 498 bp using JDP primers. 1: positive control; 2–8 samples of AK patients; 9: Non-template control; (B) The nested PCR product of 293 bp using NJK primers, 1: positive control; 2–8 positive samples of AK patients; 9: Non-template control; M: 100 bp marker.