Table 2 Comparison of IVCM, culture, PCR, and nested-PCR methods in the diagnosis of definite AK.
| Â | Definite AK Diagnosis | Â | |||
|---|---|---|---|---|---|
Positive (n = 27) | Negative (n = 15) | Total |  | Value (95% CI) | |
IVCM | |||||
Positive | 21 | 0 | 21 | Sensitivity (%) | 77.78% (59.24%−89.39%) |
Negative | 6 | 15 | 21 | Specificity (%) | 100% (79.61%−100%) |
Total | 27 | 15 | 42 | PPV (%) | 100% (84.54%−100%) |
|  |  |  |  | NPV (%) | 71.43% (50.04%−86.19%) |
Culture | |||||
Positive | 10 | 0 | 10 | Sensitivity (%) | 37.04% (21.53%−55.77%) |
Negative | 17 | 15 | 32 | Specificity (%) | 100% (79.61%−100%) |
Total | 27 | 15 | 42 | PPV (%) | 100% (72.25%−100%) |
|  |  |  |  | NPV (%) | 46.88% (30.87%−63.55%) |
One-step PCR | |||||
Positive | 17 | 0 | 17 | Sensitivity (%) | 62.96% (44.23%−78.47%) |
Negative | 10 | 15 | 25 | Specificity (%) | 100% (79.61%−100%) |
Total | 27 | 15 | 42 | PPV (%) | 100% (81.57%−100%) |
|  |  |  |  | NPV (%) | 60% (40.74%−76.6%) |
Nested PCR | |||||
Positive | 26 | 0 | 26 | Sensitivity (%) | 96.3% (81.72%−99.81%) |
Negative | 1 | 15 | 16 | Specificity (%) | 100% (79.61%−100%) |
Total | 27 | 15 | 42 | PPV (%) | 100% (87.13%−100%) |
|  |  |  |  | NPV (%) | 93.75% (71.67%−99.68%) |
