Fig. 1 | Scientific Reports

Fig. 1

From: siRNA-mediated inhibition of NTT-MMP-2 reduces oxidative stress and apoptotic signaling in an ex vivo model of ischemia/reperfusion injury

Fig. 1

Scheme of the experimental protocol. Hearts underwent 25 min of aerobic stabilization (Aero 25’), 22 min of global no-flow ischemia (Ischemia 22’), and 30 min of reperfusion at 37 °C (Reperfusion 30’). In the I/R + siRNA group, and Aero siRNA groups, MMP-2 siRNA mixed with siPORT Amine in Krebs–Henseleit buffer and in group I/R + scrRNA scrambled RNA with siPORT in K-H buffer was infused for 10 min before ischemia and again during the first 10 min of reperfusion. Coronary effluents were collected at 47 min (onset of reperfusion). Aero group was permanently perfused aerobically during the whole experiment. Figure created with BioRender.com.

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