Table 2 Ingredients composition and proximate analysis of experimental diets (% dry matter) of iron nanoparticles (Fe-NPs), fed to Pangasianodon hypophthalmus for 40 days.

From: Ecofriendly synthesis of iron nanoparticles and their role in mitigation of multiple abiotic stresses in fish

Feed ingredients

Fe-NPs-0 mg kg− 1 diet

Fe-NPs-10 mg kg− 1 diet

Fe-NPs-15 mg kg− 1 diet

Soybean meala

35.5

35.5

35.5

Fish meala

25

25

25

Groundnut meala

10

10

10

Wheat floura

17.47

17.46

17.455

Sunflower oila

4.5

4.5

4.5

Cod liver oila

1.5

1.5

1.5

CMCb

2

2

2

Vitamin and mineral mix*

2

2

2

Vitamin Cc

0.03

0.03

0.03

Lecithinb

2

2

2

Fe-NPs

0

0.001

0.0015

Proximate composition of the diets

Crude protein (CP)

35.22 ± 0.28

35.28 ± 0.03

35.05 ± 0.09

Ether extract (EE)

9.29 ± 0.04

9.54 ± 0.12

9.57 ± 0.16

Total carbohydrate (TC)

37.69 ± 0.18

37.19 ± 0.35

36.72 ± 0.45

Organic matter (OM)

90.75 ± 0.04

90.19 ± 0.49

90.29 ± 0.54

Dry matter (DM)

91.44 ± 0.20

91.82 ± 0.43

91.05 ± 0.18

Digestible energy (DE)

356.62 ± 0.82

356.67 ± 1.21

354.05 ± 1.69

Iron (Fe, mg kg− 1)

2.62 ± 0.13

13.81 ± 0.32

19.48 ± 0.60

  1. a Procured from local market, bHimedia Ltd, Himedia Ltd, cSD Fine Chemicals Ltd., India.
  2. Digestible energy (DE) (Kcal/100 g) = (% EE × 9)+(% CP × 4)+ (TC × 4).
  3. Data expressed as mean ± SE, n = 3.
  4. Composition of vitamin and mineral mixture (manual prepared mineral-vitamin mixture): vitamin B1:20 mg; vitamin B12 0.6 mcg; vitamin A 55,00,00 IU; vitamin D3 11,00,00 IU; vitamin E 75 mg; K 1,00 mg; nicotinamide 1000 mg; calcium pantothenate 2,50 mg; pyridoxine: 100 mg; I 1,00 mg; Zn 500 mg; Mn: 100 mg; Se: 2 ppm; P 30 g; Ca 50 g; Cu 200 mg; Co 45 mg.