Fig. 5 | Scientific Reports

Fig. 5

From: Mechanisms of FOXK1-regulated glycolipid metabolism in mediating TOX-induced histone lactylation to promote CD8⁺ T cell exhaustion in high-grade serous ovarian cancer

Fig. 5

Mechanism of FOXK1 promoting HGSOC immune escape in mouse epithelial ovarian cancer model (A) Endpoint tumor image (left) and tumor volume (right). Scale bar = 1 cm. (B) Observe histopathological changes in tissues through Hematoxylin and Eosin (H&E) staining (Scar bar: 50 µM). (C) Expression levels of FOXK1 and TOX in tumor tissues were analyzed by immunohistochemistry (Scar bar: 50 µM). (D) Detection of histone pan-lactic acidification levels. (E) The protein expression levels of HK2, PKM2, LDHA, Ppara, Acadm, and Decr1 were detected by Western Blot. Original blots/gels are presented in Supplementary Fig. 7. (F) Detection of Oil red O stained (Scar bar: 50 µM). (G) Testing of intracellular triglycerides, free fatty acids, and total cholesterol levels by Elisa kits. (F) Detect the expression levels of cytokines granzyme B (GzmB), IFN-γ, PRF1 and TNF-α by ELISA kits. (G) Flow cytometry analysis of the positive rate of specific cell markers. * P < 0.05, **: P < 0.01, ***: P < 0.001, n = 3.

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