Fig. 5 | Scientific Reports

Fig. 5

From: Pulsed 980 nm near-infrared photobiomodulation attenuates OVA-induced allergic asthma by modulating Th2 cytokine responses and inflammatory cell infiltration

Fig. 5

Transcriptomic analysis of the PBM effect in the OVA-allergy model. (A) Principal component analysis (PCA) of RNA-seq data from lung tissues obtained from Basal PBS, Basal OVA, PBS day 23, OVA day 23, and OVA-PBM day 23 groups. (B) Volcano plot of differentially expressed genes between the OVA day 23 and Basal PBS groups. (C) Volcano plot of differentially expressed genes between the OVA-PBM day 23 and Basal PBS groups. (D) Gene set enrichment analysis (GSEA) showing pathways upregulated in the OVA-PBM group versus the OVA group. Representative GSEA plots for (E) HALLMARK_INFLAMMATORY_RESPONSE, (F) HALLMARK_TNF_SIGNALING_VIA_NFKB, (G) Eosinophil_vs_Mac_IL25_Treatment, and (H) a heatmap of Th2-related gene sets are also presented. (I) Western blot analysis was performed to determine the levels of p-P65 and P65.Data were analyzed using an unpaired, two-tailed Student’s t-test (*P < 0.05).

Back to article page