Fig. 2 | Scientific Reports

Fig. 2

From: AsAP2 transcriptionally activates ferulate 5-hydroxylase, diverting ferulic acid metabolism toward lignin biosynthesis in Angelica sinensis.

Fig. 2

Integrated Multi-Omics Analysis in A. sinensis. (a) Volcano plot of differentially expressed genes (DEGs): Genes significantly upregulated (red) or downregulated (blue) during reproductive growth. As09G05225 (AsF5H) is highlighted as a key upregulated gene. (b) Phylogenetic tree and protein motif analysis: The phylogenetic tree illustrates the evolutionary relationships among various AsF5H gene family members from different species, including A. sinensis (Asi), Apium graveolens (Agr), Arabidopsis thaliana (Ath), Cucumis sativus (Csa), Daucus carota (Dca), Panax notoginseng (Pno), Populus trichocarpa (Ptr), Solanum lycopersicum (Sly), and Vitis vinifera (Vvi). The tree is rooted with three outgroup species. Adjacent to the phylogenetic tree is a motif analysis showing the distribution of 18 conserved motifs across the different gene family members. Each motif is represented by a colored block, with the color key provided on the right. (c) Expression heatmap: This heatmap displays the expression levels of three F5H homologs in various tissues of A. sinensis including flower, leaf, root, seed and stem. The expression levels are represented by color intensity, with darker colors indicating higher expression.

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