Fig. 3

Knockdown of PABPC5 inhibited glioma cell proliferation and migration. (A) The CCK8 assay assessed cell proliferation in PABPC5-knockdown and control U251 and U87 cell lines at designated time points (0, 24, 48, 72 h) by measuring the OD values. Proliferation curves were plotted based on these OD values. Data are presented as mean ± SD (n = 3). (B) Colony formation assays were performed to evaluate long-term proliferative ability in U251 and U87 cells. Representative images of colony formation (left) and quantification (right) are shown. The KD group of U251 and U87 cells both exhibited significantly fewer colonies than the NC group. Data are presented as mean ± SD (n = 3). (C) Migration and invasion abilities of U251 and U87 cells were assessed using Transwell assays. Representative images of the migration and invasion assays (left) and quantification of cell numbers (right) are presented. Statistically analyzed to show a significant reduction in migration/invasion ability in both cell lines KD groups. Data are presented as mean ± SD (n = 3). (D) Wound healing assays were performed on U251 and U87 cells at 0 and 24 h. Representative images of the wound healing process (left) and the quantification of wound closure (right) are shown. The KD groups inhibited the wound closure ability in both cell lines compared to the NC groups. Data are presented as mean ± SD (n = 3). (^*P < 0.05, ^**P < 0.01, ^***P < 0.001,^****P < 0.001).