Fig. 5
One-dimensional cumulative SDM representation for YKO mice. (A) Heat map of the dataset used for cumulative scoring (see Supplementary Data 5). Each SDM trait was z-score transformed and multiplied by its corrected PC1 factor loading, providing sex-axis–aligned weights. (B) Cumulative SDM scores for all WT and YKO groups. Higher values indicate a shift toward the WT-female end of the sex axis. Statistical comparisons with WT males were performed using Dunnett’s test. (C )Summary of cumulative SDM values aggregated by biological system (MP ontology). Patterns reveal gene-specific and system-specific sensitivity to Y-linked gene disruption. *corrected p < 0.05 versus WT males (B6J_M). (D) Deviation of each biological system from the WT male reference. Significant whole-body shifts (e.g., Sry KO) and distributed mild effects (e.g., Usp9y KO) are distinguished. *Corrected p < 0.05 versus WT males (B6J_M).
