Fig. 4 | Scientific Reports

Fig. 4

From: HMBOX1 plays a critical role in plumbagin-induced growth inhibition of hepatocellular carcinoma cells

Fig. 4

PLB-induced increase in HMBOX1 inhibits Huh-7 cell growth via PI3K/AKT/mTOR pathway. (A,B) Gene and protein level of HMBOX1 responded to PLB stimulation. (C,D) Representative images of cellular activities (C), colonies formation and numbers (D) in presence with PLB, HMBOX1 knockdown or not. (E) Representative images for immunoblotting and quantitative analysis for mTOR, Akt, PI3K and their phosphorylation level treated with PLB or not. (F) Representative images for immunoblotting and quantitative analysis for HMBOX1, mTOR, Akt, PI3K and their phosphorylation level treated with PLB, and/or HMBOX1-sgRNA or not. Data are presented as means ± SD, n ≥ 3, *P < 0.05 vs. control group or 0µM group, #P < 0.05 vs. PLB group.

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