Fig. 4

lncRNA PVT1 promotes cholangiocarcinoma cell proliferation, migration, and invasion by suppressing SOCS2 expression. (A–D) Western blot analysis showing increased SOCS2 expression in cholangiocarcinoma cell lines following lncRNA PVT1 silencing. (E,F) Western blot analysis of SOCS2 expression following co-transfection. (G,H) CCK-8 assay evaluating the proliferation of HuCCT1 and Hccc9810 cells after co-transfection. (I–L) Wound healing assays assessing the migration ability of HuCCT1 and Hccc9810 cells after co-transfection. (M–O) Matrix-free Transwell assays evaluating the migration capacity of HuCCT1 and Hccc9810 cells after co-transfection. (P–R) Matrigel-coated Transwell assays measuring the invasion ability of HuCCT1 and Hccc9810 cells after co-transfection.