Fig. 3 | Scientific Reports

Fig. 3

From: Cigarette smoke destabilizes G-quadruplex structures and antagonizes G4-ligand effects in macrophages

Fig. 3

CSE and RHPS4 induce DDR in non-additive manner Macrophages derived from THP-1differentiated with PMA were treated as above described and processed for IF/FISH staining with anti-γH2AX antibody and Cy3-TTAGGG PNA probe, and counterstained with DAPI. Representative images are shown in (A) at 60X magnification. Bar = 10 μm (B) The histograms report the percentage of cells displaying γH2AX foci or TIFs (γH2AX- Cy3-TTAGGG colocalization foci) in the indicated samples. The mean of three independent experiments is shown. Bars are SD. Statistically significant differences were calculated with the two-way Anova test. *=P < 0.05; ***=P < 0.001; ****=P < 0.0001. (C) The graph reports the average number of TIFs. Statistically significant differences were calculated with the Kruskal-Wallis test. ***=P < 0.001; ****=P < 0.0001.

Back to article page