Fig. 6 | Scientific Reports

Fig. 6

From: Cigarette smoke destabilizes G-quadruplex structures and antagonizes G4-ligand effects in macrophages

Fig. 6

CSE upregulates pro-inflammatory cytokines putatively through G4 destabilization. Identification of G4 putative forming sequences in the promoter regions of IL-1β (A) and TNF-α (B) with the QRGS analysis software. Analysis of IL-1β (C) and TNF-α (D) mRNA expression levels in, macrophages derived from THP-1 differentiated with PMA untreated, treated with CSE for 24 h, or with 1 µM RHPS4 for 24 h following 24 h of CSE exposure. All samples were run in triplicate, and results are shown as means ± SD. Statistical significance was calculated by Ordinary one-way Anova one-way analysis of variance followed by Tukey’s multiple comparison test. ****=P < 0.0001.

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