Fig. 3

Statistical evaluation of an association between detection of potential predators and ornamentation of guppy males. (A) Association scores between presence/absence or sequence count of each fish family and each color area of guppy males. Columns indicate six families detected as potential guppy predators and their ecological characteristics, including food habits and temporal niches. Rows indicate analyzed color spots and eDNA indicators, i.e., presence/absence and the absolute number of sequences. Each cell shows association scores: the scores before the slash symbol indicate the coefficient of logistic regression. Plus and minus scores indicate positive and negative associations, respectively. The scores after the slash indicate the exponential coefficient (eCoef) of the regression, where scores larger and lower than 1.0 indicate positive and negative associations, respectively. Significant negative and positive associations corrected for the significance level of multiple testing by the Holm method were shown by light-blue and pink shading, respectively. Gray shading shows absence of statistical significance. (B) Schematic illustration of color spot analysis of guppy males. Orange, blue-green (iridescent), and black color spots were measured as numbers of pixels by digital photographs taken at fixed distances between fish body and camera lens with a color reference card exemplified.