Table 1 Sampling site, geographic location, sampling date, and number of guppy males analyzed.

From: Estimated predator composition using environmental DNA analyses and color patterns of male guppies in introduced rivers

 

Date of samplinga/number of guppy males

Site label

Sampling site detail

Latitude/longitude

1st

2nd

3rd

4th

IRN

Natural spring named Iri-nuka

26°17’29.4"N 127°47’57.5"E

Apr. 18

May 17

May 30

Aug. 23

 

10

10

10

10

OYM

Small river in Taro farmland of Oyama

26°16’49.9"N 127°44’37.4"E

Apr. 4

May 16

May 30

Aug. 23

 

10

5

10

10

CHB

Chibu river

26°15’24.5"N 127°45’58.5"E

Apr. 4

May 17

May 30

Aug. 23

 

10

10

9

10

SNB

Artificial pond in Senbaru

26°15’02.9"N 127°45’58.9"E

Apr. 18

May 17

May 30

Sep. 6

 

10

10

10

10

URN

Makiminato river at Uranishi

26°14’57.0"N 127°44’09.8"E

Apr. 4

May 16

May 31

Aug. 23

 

10

10

10

6

OPC

Artificial pond in Okinawa Prefectural Plant Protection Center

26°12’24.6"N 127°43’10.8"E

Apr. 18

May 16

May 31

Aug. 23

 

10

10

10

10

TMY

Noha river side at Tomoyose

26°10’04.4"N 127°43’12.6"E

Apr. 18

May 16

May 31

Aug. 23

 

10

11

10

9

NSZ

Artificial river in Nishizaki reclaimed land

26°08’32.0"N 127°39’12.7"E

Apr. 4

May 16

May 31

Aug. 23

 

10

9

6

10

  1. aAll samplings were conducted in 2018.