Fig. 2

Flow cytometric gating strategy used to identify mononuclear phagocyte and lymphocyte populations. Live cells (DAPI⁻) were first selected. Doublets were removed and leukocytes (CD45⁺) were identified (A). Mononuclear phagocytes were identified as HLA-DR⁺ and lineage (CD3, CD19, CD20)⁻. pDC and DC are CD14⁻ and CD16⁻. pDC are CD123⁺ and CD11c⁻. CD141⁺DC are CD1c⁻. CD1c⁺ DC are CD141⁻ (Fig. 2Ai). T cells express CD3. B cells express CD19 and CD20. NK cells express CD56 (Fig. 2Aii). Representative histograms used to further characterise the cellular populations, from n = 1–3 experiments (B). CD14⁺ macrophages also express CD163, CD64, SIRPα/β and CD86(Fig. 2B). Fluorescence minus one (FMO) – negative control.