Fig. 1 | Scientific Reports

Fig. 1

From: LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia

Fig. 1

Overview of the LowLoad-qPCR workflow. a Schematic representation of the LowLoad-qPCR workflow using both bacterial recovery methods. After sample processing and DNA extraction, the workflow proceeds to downstream molecular analysis. b For each DNA extract obtained, two parallel molecular approaches are performed: (i) a conventional single-probe qPCR, and (ii) the LowLoad-qPCR workflow, which incorporates a random-priming amplification step followed by a multiplex TaqMan assay using multiple species-specific probes to enhance detection sensitivity.

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