Fig. 2
From: LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia
Stepwise design of the LowLoad-qPCR protocol. a Comparison of bacterial recovery efficiencies from spiked blood for E. coli, P. aeruginosa, and S. aureus using both recovery methods. b Effectiveness of DNase treatment in removing extracellular S. aureus DNA without compromising detection of intracellular E. coli DNA. c Impact of random priming amplification on total DNA yield and Cq values for all three species. d, e Cq values and fluorescence intensities benefit from probe multiplexing on a single target. c Data shown as mean ± s.d. a, b, d, e Boxes and whiskers show interquartile range and maximum and minimum values of data; centre lines in boxes represent the median. b–g Each data point represents an independent experimental replicate (n = 3 per experiment).
