Fig. 3
From: The old pea model in a new light: power of auxin over photoassimilates
Replacement of cotyledons with microtubes filled with agar, sucrose or IAA gel influences cotyledonary bud outgrowth. (a) Decotyledonated plantlet with removed apex and axillary bud bearing stem part. After this intervention one cotyledonary bud was initiated and formed a shoot. Image of plant captured 7 days after treatment. Cotyledonary replacement experiments with 200 μl microtubes in combinations (b) agar/agar, (c) agar/sucrose and (d) sucrose/sucrose. These treatments activated random outgrowth of one of the cotyledonary buds. In combinations (e) sucrose/sucrose + IAA, (f) sucrose + IAA/agar, (g) agar/IAA and (h) IAA/sucrose the cotyledonary bud outgrowth was blocked where the microtube contained IAA. In combinations (i) sucrose + IAA/sucrose + IAA, (j) sucrose + IAA/IAA and (k) IAA/IAA no cotyledonary bud outgrowth was observed. Images of plants captured 7 days after treatment. (l) Same model, 0.5% IAA paste applied on one of the cotyledonary petioles. Cotyledonary bud outgrowth was observed in axil of non-treated petiole. Image of plant captured 21 days after treatment. (n = 100 in each variant).
