Fig. 10 | Scientific Reports

Fig. 10

From: ‌PPIA regulates fatty acid and glutamine metabolism in lung adenocarcinoma based on multiomics prognostic model and experiment validation

Fig. 10

(A) Western Blot of EMT markers after interfering with PPIA. (B) Statistical plot of EMT markers in A549 cells and NCI-H1975 cells after interfering with PPIA. (C) Quantification of relative glutamine uptake following PPIA knockdown in A549 and NCI-H1975 cells. (D) Quantification of relative Alpha Ketoglutarate production following PPIA knockdown in A549 and NCI-H1975 cells. (E) Quantification of GSH/GSSG ration following PPIA knockdown in A549 and NCI-H1975 cells. (F) Quantification of FFA following PPIA knockdown in A549 and NCI-H1975 cells. (G) Western blot analysis of c-Myc protein expression in A549 and NCI-H1975 cells following PPIA knockdown and/or Myc overexpression. (H, J) RT-qPCR analysis of c-Myc mRNA expression in A549 and NCI-H1975 cells after PPIA knockdown and/or Myc overexpression. (I) Quantification of c-Myc protein levels based on Western blot results in A549 and NCI-H1975 cells.

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