Fig. 1 | Scientific Reports

Fig. 1

From: Single-cell transcriptomics and spatial validation reveal dysfunction of keratinocytes and NK cells driving acne development

Fig. 1

Single-cell transcriptomic analysis reveals dynamic remodeling of immune-epithelial structures during acne progression. (A) Workflow for single-cell RNA sequencing from skin biopsies, including tissue dissociation, single-cell suspension preparation, library preparation, and sequencing. (B) UMAP plot showing 19 transcriptionally distinct clusters across all samples, each labeled with a unique color. (C) UMAP plot colored by major cell lineages, including keratinocytes, NK cells, fibroblasts, monocytes/macrophages, endothelial cells, B cells, and neutrophils. (D) Cell type composition cross-section illustrating cellular composition across NC, MA, and SA, highlighting NK cell enrichment in MA and keratinocyte expansion in SA. (E) Stage-specific UMAP plots (NC, MA, SA) revealing cellular trajectories from NK cell-driven immune activation to keratinocyte-driven pathological remodeling.

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