Fig. 3 | Scientific Reports

Fig. 3

From: LBPs NPs suppress breast cancer progression by inhibiting YAP1 expression to induce ferroptosis and alter energy metabolism

Fig. 3The alternative text for this image may have been generated using AI.

LBPs suppress YAP1-overexpression-driven malignant progression of breast cancer cells. (A) RT-qPCR analysis of the relative mRNA expression of YAP1 in MDA-MB-231 cells after transfection with YAP1-siRNA. Data are presented as the mean ± SD (n = 3), *** P < 0.001, ** P < 0.01. (B,C) Colony formation assays were used to evaluate the proliferative capacity of MDA-MB-231 cells after LBPs treatment or transfection with a YAP1-overexpression plasmid or YAP1-siRNA. Data are presented as the mean ± SD (n = 3), *** P < 0.001. ns: not significant. (D,E) Representative TUNEL staining images of MDA-MB-231 cells after LBPs treatment, transfection with the YAP1-overexpression plasmid, or YAP1-siRNA. Red fluorescence indicates TUNEL-positive cells and blue fluorescence denotes nuclei. Data are presented as the mean ± SD (n = 3), *** P < 0.001, ** P < 0.01, ns: not significant. Scale bar, 200 μm. (F,G) Actin-Tracker Green-488 probes were employed to visualize F-actin architecture in MDA-MB-231 cells after LBPs treatment, transfection with the YAP1-overexpression plasmid, or YAP1-siRNA. Data are presented as the mean ± SD (n = 3), *** P < 0.001. ns: not significant. Scale bar, 50 μm. (H,I) Transwell assays were performed to evaluate the invasive capacity of MDA-MB-231 cells after LBPs treatment, transfection with the YAP1-overexpression plasmid, or YAP1-siRNA. Data are presented as the mean ± SD (n = 3), *** P < 0.001, ** P < 0.01, ns: not significant. Scale bar, 200 μm.

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