Table 2 Comparison between gene sequencing and MALDI biotyper identification among Legionella species.
mip and rpoB gene sequencing identification | L. anisa 36/104 (34.61%) | L. londiniensis 6/104 (5.77%) | L. nautarum 20/104 (19.23%) | L. rubrilucens 31/104 (29.81%) | L. taurinensis 10/104 (9.62%) | L. pneumophila 1/104 (0.96%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MALDI-TOF MS | DS | EDS | E | DS | EDS | E | DS | EDS | E | DS | EDS | E | DS | EDS | E | DS | EDS | E |
Species level (High confidence score) | 35/36 (97.22%) | 36/36 (100%) | 36/36 (100%) | 3/6 (50%) | 2/6 (33.33%) | 3/6 (50%) | 16/20 (80%) | 15/20 (75%) | 15/20 (75%) | 3/31 (9.68%) | 5/31 (16.13%) | 7/31 (22.58%) | 10/10 (100%) | 9/10 (90%) | 9/10 (90%) | 1/1 (100%) | 1/1 (100%) | 1/1 (100%) |
Genus level (Low confidence score) | 1/36 (2.78%) | 3/6 (50%) | 4/6 (66.67%) | 3/6 (50%) | 4/20 (20%) | 5/20 (25%) | 5/20 (25%) | 25/31 (80.65%) | 24/31 (77.42%) | 19/31 (61.29%) | 1/10 (10%) | 1/10 (10%) | ||||||
No identification | 3/31 (9.68%) | 2/31 (6.45%) | 5/31 (16.13%) | |||||||||||||||
