Fig. 3
From: S100 calcium-binding protein A8 exacerbates deep vein thrombosis in vascular endothelial cells
RNA levels of NF-kb(RelA), NLRP3, Caspase-1, GSDMD and IL-1beta in human umbilical vein endothelial cells (HUVECs) after intervention: (a) The 5 µg/mL and 10 µg/mL cohorts and the control group showed a significant difference in RelA RNA concentrations, whereas the discrepancy from the 2 µg/mL group was minimal. Furthermore, the S100A8 + MCC950 cohort exhibited notable fluctuations compared with the control group, unlike the 10 µg/mL cohort. (b) Significant differences were observed in NLRP3, caspase-1, and GSDMD RNA concentrations across all groups compared with the control group. Notably, a substantial gap in these differences was observed in the S100A8 + MCC950 group versus the 10 µg/mL group. (c) There was a significant variation in IL-1β RNA concentrations among the 5 µg/mL, 10 µg/mL, and S100A8 + MCC950 groups compared with the control. However, this difference was not significant in the 2 µg/mL group. Consequently, there was a significant difference between the S100A8 + MCC950 and 10 µg/mL groups. ***P < 0.001, **P < 0.01, *P < 0.05, ns P > 0.05.
