Fig. 2 | Scientific Reports

Fig. 2

From: Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches

Fig. 2

Xoo distribution routes in rice plants and Xoo exudation to soil and water in PSL2 and PSL2-Xa21 rice varieties after 14 and 28 days of infection (14–28 DAI). The rice tissues used for Xoo detection included unclipped new leaves (Unclipped-N), unclipped old leaves (Unclipped-O), clipped leaves, stem, root, soil and water. Detection of Xoo infection by PCR technique (A) and cLAMP technique (B) analyzed by the amounts of bacteria in the samples using quantification PCR (qPCR) (C). Diagram of Xoo infection levels from rice leaves to environments nearby based on PCR, cLAMP, and qPCR techniques (D). The unclipped leaves were used as the control (CT) and the water-clipped leaves were used as the mock control (WC). The positive control (+) was genomic DNA of Xoo. Non-DNA template was used as negative control (NC) to determine whether the reaction was contaminated. The grouping of gels was cropped from different gels and their original images are in Supplementary Information 1.

Back to article page