Table 2 Primer sequences for DNA amplification of Xoo by cLAMP and PCR.
Method | Gene | LAMP primer | Oligonucleotide (5′–3′) | Size (bp) | References |
|---|---|---|---|---|---|
cLAMP | Xoo4009 | LpXoo4009F3 | CGCCCCATGAAGAACTTCG | 210 | |
LpXoo4009B3 | GTTCCGGTGTGGTGGTTC | Â | Â | ||
LpXoo4009FIP | ACTTGCGCATCAATGATCCCGTTTTTGGGCCGTAGCAGTCGT | Â | Â | ||
LpXoo4009BIP | CACCTGCCGCAGATCCGTTTTTGCGATCTCAAAGGCCGTCA | Â | Â | ||
PCR and qPCR | Hypothetical protein | Xoo4009F | GTTCACCCTGCCCTTCATTTCCGTCGTCATC | 302 | |
(PXO_03999) | Xoo4009R | CCTTTGAGATCGCATGCATGAAGAACCACCACA | Â | Â |
