Fig. 2 | Scientific Reports

Fig. 2

From: The compensatory enrichment of sphingosine-1-phosphate on HDL in FSGS enhances the protective function of glomerular endothelial cells compared to MCD

Fig. 2

FSGS-HDL restores HRGECs function due to its phospholipid composition. (a,b) Western blot analysis of p-eNOS level in HRGECs cocultured with various groups of apoHDLs at 30 µg/ml for 6 h. (c,d) The immunofluorescence images and quantitative analysis exhibit the intracellular NO levels across different cell groups, which have been detected utilizing a NO bioprobe. (e,f) Western blot analysis of p-eNOS level in HRGECs cocultured with various groups of pho-HDLs at 30 µg/ml for 6 h. (g,h) Using a NO-sensitive fluorescent probe, the intracellular NO expression in different cell groups was detected and analyzed (Scale bar: 100 μm). Data presented as mean ± SEM, with significance levels marked as *P < 0.05, **P < 0.01, ***P < 0.001. Statistical analysis employed one-way ANOVA. All experiments were replicated three times.

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