Fig. 1
FRET measurement of the DNA unwrapping of NCPs with different DNA sequences at the entry site. (a) The DNA sequences and the positions of fluorescent dyes for the FRET analysis are shown in the left panel. Green and yellow stars indicate the positions of the donor and acceptor dyes, respectively. The distribution of fluorescent dyes, calculated by FPS software25, are denoted as green and yellow dots with the structure of the nucleosome (PDB ID: 3lz0) in the middle and the right panel. The positions mutated to AA/TT or TA repeats are denoted in red in the middle panel. In the right panel, AA/TT repeats and C/G of Imperfect AA NCP are shown in red and cyan, respectively. (b) The mean distance and standard deviation of the distances obtained from the FRET measurement at 0 mM KCl. (c,d) The mean distance (c) and standard deviation (d) of the distances titrated by KCl for NCPs. The inset graph shows the zoom-in of the 0–300 mM range.
