Fig. 2 | Scientific Reports

Fig. 2

From: FRET analysis of the unwrapping of nucleosomal DNA containing a sequence characteristic of the + 1 nucleosome

Fig. 2

Ethidium-induced nucleosome disruption. (a) Structure of the 601 NCP (PDB ID: 3lz0). The entry/exit site of 601 NCP was mutated to either AA or TA repeat sequence and is shown in red. (b) Fractions of the intact NCPs (θ) after five hours of incubation of 601, AA, and TA NCP with different concentrations of ethidium. The mean value and the standard deviation obtained from the three repetitive experiments are shown. (c) Representative native PAGE gel images. The band of intact AA NCPs disappears more quickly than those of 601 and TA NCPs. The images were cropped. Whole gel images for three repetitive experiments are shown in Fig. S5.

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