Fig. 6 | Scientific Reports

Fig. 6

From: Selective toxicity of a novel antimicrobial peptide Acidocin 4356 against Pseudomonas aeruginosa and Acinetobacter baumannii in human cell-based in vitro infection models

Fig. 6

Comparative Analysis of the toxicity of ACD at 60 µg/mL concentration on Calu-6 and THP-1 cell lines in monoculture and co-culture conditions following a 3-hour treatment period. (a) Flow cytometry chart depicting the toxicity of ACD on Calu-6 and THP-1 cell lines in co-culture and monoculture conditions. While Calu-6 cells show no significant difference in viability between co-culture and monoculture, THP-1 cells exhibit enhanced viability when treated with ACD in co-culture compared to monoculture. (b) Comparing the viability of Calu-6 and THP-1 cells in monoculture and co-culture conditions. There is a significant difference between THP-1 treated in monoculture condition and control, THP-1 treated in monoculture condition, and THP-1 treated in co-culture condition. No significant difference is observed in Calu-6 cell survival between the two conditions. The data are based on three independent experiments, and two replicates were used for each variation. **0.001 > P < 0.01, *0.01 > P < 0.05 (one-way ANOVA).

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