Fig. 5 | Scientific Reports

Fig. 5

From: CNS resident macrophages exhibit region-specific states and immunogenic responses during Rbpj-deficient brain arteriovenous malformation

Fig. 5

Increased density of Iba1 + /CD68 + microglia was associated with areas of increased vascular permeability in P14 Rbpj-mutant brain tissue. (A) Schematic depicts midsagittal cortical region used in analyses. (B–E) Immunostaining against Iba1 (red) and CD68 (white) in control (B, D) and mutant (C, E) cortex at P14 demonstrated increased density of Iba1 + /CD68 + microglia in areas of increased vascular extravasation of 4 kDa fluorescein-conjugated Dextran (green). DAPI labeled cell nuclei (blue). (F) Schematic representation of the midsagittal cerebellum regions. (G–J) Immunostaining against Iba1 (red) and CD68 (white) in control (G, I) and mutant (H, J) cerebellum at P14 demonstrated increased density of Iba1 + /CD68 + microglia in areas of increased vascular extravasation of 4 kDa fluorescein-conjugated Dextran (green).

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