Fig. 2

CCN1 transfection efficiency. (A) Western blot analyses show CCN1 protein expression levels in OE19 and KYSE150 cells after transfection with pcDNA3.1-CCN1 (pCCN1) compared to vector-transfected control. (B) Quantitative analysis of CCN1 expression in OE19 and KYSE150 in response to cell transfection, based on at least 3 replicates. (C) Western blot analyses show CCN1 protein expression levels in OE33 and KYSE410 cells after transfection with pcDNA3.1-CCN1 (pCCN1) compared to vector-transfected control. (D) Quantitative analysis of CCN1 expression in OE33 and KYSE410 in response to cell transfection, based on at least 3 replicates. *Statistical significance. M – molecular weight ladder.