Table 1 Newly designed PCR-based markers for DArT-seq and silicoDArT loci from recent genome-wide association study46 and allele count observed in white lupin germplasm panel.
Marker name | Enzyme | Reference allele products (bp) | Alternative allele products (bp) | Reference allele count | Heterozygote count | Alternative allele count |
|---|---|---|---|---|---|---|
Chr02_2625564_D_dCAPS | HpaII | 25, 113 | 138 | 298 | 0 | 2 |
Chr06_14434379_CAPS | HpaII | 558 | 254, 334 | 277 | 7 | 16 |
Chr07_16560064_D_CAPS | HpyCH4IV | 14, 478 | 14, 179, 299 | 165 | 18 | 117 |
Chr08_3090075_D_dCAPS | NlaIV | 21, 202 | 223 | 129 | 17 | 153 |
Chr08_12044717_CAPS | AcuI | 181 | 77, 104 | 296 | 0 | 4 |
Chr11_5890565_D_dCAPS | MaeII | 7, 134 | 7, 18, 116 | 295 | 0 | 5 |
Chr11_14834409_CAPS | AciI | 90, 91 | 181 | 293 | 2 | 5 |
Chr13_12561729_D_PCR | – | – | 129 | 131 | 3 | 166 |
Chr13_1469866_CAPS | RsaI | 127, 333 | 127, 132, 201 | 270 | 13 | 17 |
Chr16_572706_CAPS | HaeIII | 35, 128 | 163 | 219 | 28 | 53 |
