Fig. 4
Changes in the gene expression of programmed cell death-related genes. (a) Results from the quantitative real-time PCR of key factors involved in the apoptosis and necrosis pathways, namely, RIPK1, RIPK3, MLKL, TRAF-2, p53, Bax, Bcl2, FADD, and PARP1, in the ileum. All the data are presented as the means ± SEMs (n = 5). ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001 compared with each corresponding control group. (b) Relative mRNA expression level of Bax. The ratio of Bax/Bcl-2 was greater in all NEC groups than in the corresponding control groups. The values represent the means ± SEMs (n = 5) for each group. ns = not significant and *p < 0.05 NEC versus control.
