Fig. 3

Increasing NO production-induced changes in urinary sodium excretion kinetics and diurnal polyuria index. 24-hour sodium excretion (a), sodium excretion during the active (b), and sodium excretion during inactive periods (c) were compared between the Sed and Mod-Ex groups. A two-tailed Student’s t-test was performed (n = 6 per group); *P < 0.05, **P < 0.01. Error bars represent s.e.m., n.s.: Not significant. The 4-hour urinary volume for 3.5 consecutive days in the Sed (d) and Mod-Ex (e) groups at week 2 (baseline) and week 6 was measured using the automated voiding staining on the paper method (aVSOP). Changes in the DPi were compared between Sed and Ex groups (f). Data were obtained from the same mice. Two-way repeated-measures analysis of variance (ANOVA) was performed (n = 6 per group); *P < 0.05 (time-by-group interaction). Error bars represent s.e.m.