Fig. 8
From: Assessment of Hsp90β-selective inhibitor safety and on-target effects
Western blots of MCF-10A, MDA-MB-231, and MDA-MB-468 cells after 24-hour treatment with NDNB1182. 100 nM pan-inhibitor AUY922 and 0.1% DMSO were used as positive and negative controls. Hsp90β-selective inhibitor NDNB1182 induces expression of Hsp70 in normal (MCF-10A) and triple-negative breast cancer (MDA-MB-231, MDA-MB-468). However, a significant induction of Hsp90α was only observed in the normal MCF-10A cells and not in either of the breast cancer cell lines. Bands were derived from the same experiment and duplicate blots that were run in parallel.
