Fig. 3 | Scientific Reports

Fig. 3

From: Intratympanic administration of dexamethasone attenuates radiation induced damage to middle ear mucosa

Fig. 3

Analysis of the time and concentration dependence of the cytotoxicity induced by DEX and radiotherapy in human middle ear epithelial (HMEE) cells. (A) Experimental timeline of radiotherapy and dexamethasone treatment of HMEE cells. (B) HMEE cells plated in 24-well plates were incubated in media supplemented with different concentrations (0, 10, 25, 50, or 100 µg/mL) of DEX for various durations. Cell viability was measured via a Cytotox Green assay. (C) HMME cells were irradiated (10–20 Gy), and the IncuCyte® system was used for real-time monitoring of the effects of radiotherapy on the green area/phase area. The data represent the means ± S.E.M.s of three independent experiments. Two-way ANOVA with Tukey’s multiple comparisons test; ****p < 0.0001.

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