Fig. 4 | Scientific Reports

Fig. 4

From: Intratympanic administration of dexamethasone attenuates radiation induced damage to middle ear mucosa

Fig. 4

Dexamethasone reduces the extent of HMEE cell death induced by radiotherapy (RTx). (A) Images of HMEE cells preincubated with 25 µg/mL DEX before exposure to RTx for 30 min, followed by staining with IncuCyte® Cytotox Green. Compared with that in cells incubated for 24 h (control), green fluorescence was significantly increased following exposure to RTx (10 Gy) at 48 h and 72 h. Cytotox Green confluence after RTx treatment was reduced in the presence of DEX. Scale bar = 300 μm. (B) Graphs indicate a time-dependent increase in green fluorescence (cell death)/phase area for 72 h. All data represent the means ± S.E.M.s of three independent experiments. Two-way ANOVA with Tukey’s multiple comparisons test; **p < 0.01, ****p < 0.0001.

Back to article page