Fig. 1

Pedigree and confirmation of the SERPINB4 gene variant identified in twin patients with Crohn’s disease. (A) Pedigree of the twin patients with Crohn’s disease, “white square box” represents healthy males, “white circle” represents healthy females, and “black filled square box” represents male patients. (B) Sequencing chart for the SERPINB4 gene mutation, showing the A-to-G mutation at the 478th base. (C–D) The mutation caused the 160th amino acid to change from AAA (lysine) to GAA (glutamic acid); prediction of the protein architecture, made using Alpha Fold3 software. (E) Effect of SERPINB4 expression on the survival rate of patients with colorectal adenocarcinoma (COAD). (F) Volcano plot showing the effects of high SERPINB4 expression in intestinal epithelial cells after stimulation, according to the GEO database. (G) Effects of the high SERPINB4 expression in intestinal epithelial cells following stimulation, according to the GEO database.