Fig. 4

Genotyping of the Culex quinquefasciatus from Abu Dhabi, UAE, based on the kdr mutation L1014F using DNA sequencing. Sequences were aligned with the genomic reference sequence (GenBank accession number NC_051862.1) using Unipro UGENE software. The chromatograms were viewed using SnapGene software (www.snapgene.com). The mutation occurs at position number 193,055. Susceptible (SS) insects have the sequence TTA; Resistant homozygous (RR) insects have the sequence TTT; and heterozygous (RS) insects have the sequence TTW, where W is T/A. Chromatograms show a single peak in SS and RR genotypes and two peaks in RS genotypes at the kdr mutation position. kdr, Knockdown resistance; RR, homozygous resistant (kdr/kdr); RS, heterozygous (kdr/sus); SS, homozygous susceptible (sus/sus).