Table 2 Primers used for Culex quinquefasciatus identification and kdr mutation detection in Abu Dhabi, UAE.

From: The southern house mosquito Culex quinquefasciatus in Abu Dhabi, UAE, is developing resistance to deltamethrin insecticide

Primer name

Direction

Primer sequence (5′ to 3′)

PCR product size (bp)

Used for

References

ACEquin

For

CCTTCTTGAATGGCTGTGGCA

274

Identification

75

B1246s

Rev

TGGAGCCTCCTCTTCACGG

Ā 

Identification

Ā 

LCO1490

For

GGTCAACAAATCATAAAGATATTGG

710

Identification

76

HCO2198

Rev

TAAACTTCAGGGTGACCAAAAAATCA

Ā 

Identification

Ā 

Cq1

For

GTGGAACTTCACCGACTTC

380

AS-PCR

34

Cq2

Rev

GCAAGGCTAAGAAAAGGTTAAG

Ā 

AS-PCR

Ā 

Cq3

For

CCACCGTAGTGATAGGAAATTTA

380

AS-PCR

34

Cq4

For

CCACCGTAGTGATAGGAAATTTT

Ā 

AS-PCR

Ā 

IIP_F

For

GGTGGAACTTCACCGACTTC

676

Sequencing

77

IIS6_R

Rev

GGACGCAATCTGGCTTGTTA

Ā 

Sequencing

Ā 
  1. AS-PCR = Allele-specific PCR, For = forward, Rev = reverse.