Fig. 2
Increased LD-Mito contacts in isolated primary hepatocytes and hepatic tissue in db/db mice livers. (a) Representative images of mitochondria and LDs in primary hepatocytes are shown (scale bars = 20 μm). LDs were marked by the neutral BODIPY 493/503 fluorescent dye (BODIPY) and mitochondria by MitoTracker deep red dye (MitoTracker). (b) Percentage area of lipid droplets in primary hepatocytes. (c) TEM images of lipid droplets and mitochondria in the liver. Red, mitochondria; green, LDs. (d) Percentage of total mitochondria in contact with lipid droplets. (e) The ratio of the length of LD-Mito contacts to the perimeter of LD. (f) The ratio of the length of LD-Mito contact to the perimeter of mitochondria. Data are mean ± SEM. n = 3 (2–3 images analyzed per sample for fluorescent images and micrographs). Student’s t-test was performed. *P < 0.05, **P < 0.01.
