Fig. 2

Phylogenetic analysis of amplicon sequences obtained by tNGS Neighbor-Joining trees of 16 S rRNA gene sequences from one positive human sample (Ocoy 136, Fig. 2 panels A and B) and an UPGMA tree of RNase P gene sequences from three positive human samples (Ocoy 136, KOP-02, KOP-08, Fig. 2 panel C) showed the sequences grouped with Mycoplasma haemocanis. Portions of the 16 S rRNA gene sequence were identical between M. haemocanis and M. haemofelis (Fig. 2 panel B); thus, use of the RNase P gene was better to distinguish between these two species (Fig. 2 panel C).