Table 1 Components of JFBX absorbed in the blood (component classification: (A) flavonoids, (B) organic acids, (C) terpenes, (D) alkaloids, (E) Coumarins, and (F) others. *Compounds were identified by comparison with a reference.
No. | Name of components | Retention time (min) | Theoretical value (DA) | Actual value (m/z) | Deviation (ppm) | Ion mode | MS2 | Component classification |
|---|---|---|---|---|---|---|---|---|
1 | DL-Pyroglutamic acid | 1.0093 | 129.0426 | 130.0495 | -3.0187 | +H | 116,84 | D |
2 | *Paeoniflorin | 1.0146 | 462.1526 | 463.1617 | 3.8561 | +H | 381,325,279,179,151 | C |
3 | Tetrahydropalmatine | 1.0238 | 355.1784 | 356.1848 | -2.2126 | +H | 177 | D |
4 | Albiflorin | 1.6690 | 480.1632 | 481.1711 | 1.3926 | +H | 417,255,179,105 | C |
5 | *Chlorogenic acid | 8.5547 | 354.0951 | 353.0885 | 2.0725 | -H | 336 | B |
6 | Umbelliferone | 8.7037 | 162.0317 | 163.0382 | -4.8414 | +H | 150,137 | E |
7 | Calycosin 7-O-glucoside | 9.1631 | 446.1213 | 447.1284 | -0.4426 | +H | 429,327 | A |
8 | Phthalic acid | 9.3837 | 166.0266 | 165.0198 | 2.7624 | -H | 120,75 | B |
9 | Sinomenine | 10.2512 | 329.1627 | 330.1697 | -0.9166 | +H | 294 | E |
10 | Paeonilactone C | 11.2016 | 318.1103 | 319.1166 | -3.0876 | +H | 211,108 | E |
11 | Paeonilactone-B | 11.2017 | 196.0736 | 197.0801 | -3.7210 | +H | 180,135 | E |
12 | Puerarin 4’-O-glucoside | 11.3261 | 578.1636 | 579.1707 | -0.1631 | +H | 560,543 | A |
13 | Puerarin 6″-O-xyloside | 12.6857 | 548.1530 | 549.1601 | -0.3373 | +H | 530,513 | A |
14 | Cyclanoline | 13.2214 | 342.1705 | 342.1692 | -2.2389 | -e | 192 | D |
15 | Scopoletin | 14.2234 | 192.0423 | 193.0487 | -4.3719 | +H | 176,159,114 | F |
16 | 3’-Methoxypuerarin | 14.6883 | 446.1213 | 447.1280 | -1.3178 | +H | 429,411,393,297,102 | A |
17 | Glutinoside | 15.5202 | 398.0980 | 399.1070 | 4.3197 | +H | 219,187 | E |
18 | 4-O-Galloylalbiflorin | 15.9923 | 632.1741 | 633.1817 | 0.4158 | +H | 363,346 | C |
19 | *Fangchinoline | 16.1631 | 608.2886 | 609.2966 | 1.0726 | +H | 566,367,191 | D |
20 | 3’-Hydroxy puerarin | 16.4986 | 432.1056 | 433.1126 | -0.8463 | +H | 253 | D |
21 | Formononetin 7-O-Glucoside | 17.2070 | 430.1264 | 429.1195 | 0.8333 | -H | 249,221 | A |
22 | 4’-O-Methylpuerarin | 17.3766 | 430.1264 | 431.1332 | -1.0570 | +H | 251,223 | A |
23 | *Tetrandrine | 19.4570 | 622.3043 | 623.3116 | 0.1295 | +H | 580,381,122 | D |
24 | 7,3’,4’-Trihydroxyflavone | 19.9529 | 270.0528 | 271.0589 | -4.2747 | +H | 255,238 | A |
25 | Butin | 20.2586 | 272.0685 | 273.0749 | -3.1885 | +H | 257,240,223 | A |
26 | *Kaempferol | 21.4618 | 286.0477 | 287.0541 | -3.2422 | +H | 255,111 | A |
27 | Nordalbergin | 21.5405 | 254.0579 | 255.0650 | -0.8800 | +H | 239,222,177 | A |
28 | 7-Methoxy-3,6,3’,4’-tetrahydroxyflavone | 21.6469 | 316.0583 | 317.0648 | -2.5584 | +H | 289 | A |
29 | Isorhamnetin | 21.6469 | 316.0583 | 317.0648 | -2.5584 | +H | 289,256 | A |
30 | Chrysoeriol | 21.7557 | 300.0634 | 301.0697 | -3.3054 | +H | 273,240 | A |
31 | Dalbergin | 22.2075 | 268.0736 | 269.0799 | -3.4893 | +H | 253,236 | F |
32 | Formononetin | 22.2075 | 268.0736 | 269.0799 | -3.4893 | +H | 253 | A |
33 | Hesperetin | 22.3001 | 302.0790 | 303.0852 | -3.8362 | +H | 275,242 | A |
34 | 5,7-Dihydroxyflavanone | 22.4728 | 256.0736 | 257.0796 | -4.9319 | +H | 241,224 | A |
35 | Chrysophanol | 22.8396 | 254.0579 | 253.0513 | 2.7308 | -H | 236,220 | F |
36 | Daidzein | 22.8894 | 254.0579 | 255.0643 | -3.4554 | +H | 237,145,137 | A |
37 | Bowdichione | 23.0140 | 298.0477 | 299.0541 | -2.9332 | +H | 283,271 | D |
38 | 3’-Hydroxymelanettin | 23.4157 | 300.0634 | 301.0696 | -3.6196 | +H | 285 | A |
39 | Xenognosin B | 23.5508 | 284.0685 | 285.0747 | -3.5430 | +H | 270,137 | F |
40 | Dimethyl camphorate | 23.5797 | 228.1362 | 227.1296 | 3.3229 | -H | 185,144 | F |
41 | Stephanthrine | 23.6127 | 293.1416 | 294.1480 | -3.0361 | +H | 253 | E |
42 | *Benzoylpaeoniflorin | 23.8816 | 584.1894 | 585.1970 | 0.6346 | +H | 315 | C |
43 | Carvacryl acetate | 24.0161 | 192.1150 | 193.1217 | -2.9164 | +H | 148 | F |
44 | sedanoic acid | 24.0210 | 210.1256 | 211.1321 | -3.5731 | +H | 192 | B |
45 | Violanone | 24.0589 | 316.0947 | 317.1009 | -3.3293 | +H | 163 | A |
46 | Nepetoidin B | 24.1102 | 314.0790 | 315.0854 | -2.9836 | +H | 299 | F |
47 | Naringenin | 24.1893 | 272.0685 | 273.0747 | -3.6971 | +H | 137 | A |
48 | Liriodenine | 24.1904 | 275.0582 | 276.0647 | -3.0939 | +H | 247,231 | D |
49 | 9-O-Methylcoumestrol | 24.2079 | 282.0528 | 283.0590 | -3.7701 | +H | 267,250 | E |
50 | 3′-Methoxydaidzein | 24.2107 | 284.0685 | 285.0747 | -3.7314 | +H | 267,175 | A |
51 | Angelicone | 24.3009 | 288.0998 | 287.0924 | -0.2029 | -H | 270,207 | F |
52 | Baicalein | 24.3481 | 270.0528 | 271.0589 | -4.2864 | +H | 255,238,221 | A |
53 | Biochanin A | 24.4884 | 284.0685 | 285.0746 | -4.0101 | +H | A | |
54 | Stevein | 24.4884 | 284.0685 | 285.0748 | -3.2293 | +H | 255,149 | A |
55 | Paeonol | 24.7965 | 166.0630 | 167.0695 | -4.4024 | +H | 150,133,90 | F |
56 | Melilotocarpan C | 24.8583 | 330.1103 | 331.1171 | -1.6974 | +H | 267,149,133 | E |
57 | Cyperolone | 24.8635 | 236.1776 | 237.1838 | -4.6272 | +H | 81 | E |
58 | Senkyunolide F | 24.9114 | 206.0943 | 205.0878 | 3.7085 | -H | 188,143 | F |
59 | (Z)-3-Butylidenephthalide | 24.9293 | 188.0837 | 189.0903 | -3.8651 | +H | 172 | E |
60 | Isodalbergin | 24.9395 | 268.0736 | 269.0799 | -3.4178 | +H | 253,241 | A |
61 | Senkyunolide D | 24.9930 | 222.0892 | 221.0827 | 3.2470 | -H | 204,158 | F |
62 | Vallesiachotamine | 25.0750 | 350.1630 | 351.1693 | -2.7774 | +H | 146 | D |
63 | Methylnissolin | 25.1866 | 300.0998 | 301.1057 | -4.5801 | +H | 264,172 | E |
64 | Phenylacetic acid | 25.3869 | 136.0524 | 137.0592 | -3.9326 | +H | 92 | B |
65 | 2’-Hydroxyacetophenone | 25.3869 | 136.0524 | 137.0592 | -3.9326 | +H | 121,91 | F |
66 | Isochavibetol | 25.5398 | 164.0837 | 165.0907 | -2.0258 | +H | 135,101 | F |
67 | Isocurcumenol | 25.8572 | 234.1620 | 235.1684 | -3.7746 | +H | 219 | F |
68 | alpha-Cedrene | 25.9233 | 204.1878 | 205.1942 | -4.3809 | +H | 177 | F |
69 | Calacorene | 25.9312 | 200.1565 | 201.1630 | -3.8946 | +H | 159 | F |
70 | *Caffeic acid | 25.9379 | 180.0423 | 181.0487 | -4.5240 | +H | 162,109 | B |
71 | Jolkinolide E | 26.0300 | 300.2089 | 301.2149 | -4.3336 | +H | 256 | E |
72 | Medroxyprogesterone | 26.2373 | 344.2351 | 345.2414 | -2.8774 | +H | 291 | E |
73 | Senkyunolide G | 26.3370 | 208.1099 | 209.1166 | -3.1262 | +H | 192,147 | F |
74 | (+)-alpha-Pinene | 26.6546 | 136.1252 | 137.1318 | -4.6733 | +H | 123 | F |
75 | alpha-Bergamotenol | 26.7282 | 220.1827 | 221.1894 | -2.8775 | +H | 205 | F |
76 | Phlorol | 26.8093 | 122.0732 | 123.0804 | -0.0632 | +H | 110 | F |
77 | Riligustilide | 26.8380 | 380.1988 | 381.2051 | -2.4020 | +H | 185,109,95 | F |
78 | 1,3,8-p-Menthatriene | 26.8411 | 134.1096 | 135.1162 | -4.6493 | +H | 96 | F |
79 | 2-Ethyl-hexenal | 26.8443 | 126.1045 | 127.1114 | -2.9840 | +H | 100 | F |
80 | Rosifoliol | 26.8628 | 222.1984 | 223.2052 | -1.9626 | +H | 206 | F |
81 | alpha-Phellandrene | 27.0859 | 136.1252 | 137.1320 | -3.3549 | +H | 109,83,71 | F |
82 | 6-Butyl-1,4-cycloheptadiene | 27.0895 | 150.1409 | 151.1478 | -2.2346 | +H | 136 | F |
83 | (-)-Cyperenoic acid | 27.1739 | 248.1776 | 249.1846 | -1.0367 | +H | 205 | B |
84 | 11-Dodecenoic acid | 27.1944 | 198.1620 | 199.1701 | 4.0089 | +H | 154 | B |
85 | alpha-Cyperone | 27.2110 | 218.1671 | 219.1743 | -0.3993 | +H | 121,93 | F |
86 | Kudzusapogenol A | 27.7038 | 490.3658 | 491.3750 | 3.7720 | +H | F | |
87 | 3-Butylidene-7-hydroxyphthalide | 27.8040 | 204.0786 | 205.0856 | -1.7880 | +H | 188,143 | F |
